UCI
School of Biological Sciences
Developmental and Cell Biology

David Gardiner

David Gardiner, PhD

David Gardiner, Ph.D.
Professor

4111 Natural Sciences II
University of California Irvine
Irvine, CA 92697-2305

Tel: (949) 824-2792
Fax: (949) 824-4709
Email:dmgardin@uci.edu
Website: Lab Homepage

Limb development and regeneration - How to Regenerate a New Leg: What we can learn from salamanders - Alone among vertebrates, urodele amphibians are able to regenerate lost body parts as adults. The key to this ability is that limb cells are triggered to dedifferentiate and reinitiate growth and pattern formation. Our strategy is to use axolotls (Ambystoma mexicanum) to discover the signals that trigger the regeneration response, in the belief that these signals have enormous potential and consequences for human health. Our long term goals are to identify the regeneration-enabling signals in limbs, in order to support progress towards the eventual application of these molecules to the improvement of human repair mechanisms. In current research, we are using assays derived from the extensive experimental history of regenerating limbs, to test the roles of several signaling molecules known to be essential for limb development …

Recent Publications

  • Satoh, A., Bryant, S.V. and Gardiner, D.M. (2008). Regulation of dermal fibroblast dedifferentiation and redifferentiation during wound healing and limb regeneration in the Axolotl. Dev. Growth & Diff. (in press)
  • Satoh, A., Graham, G.M.C., Bryant, S.V. and Gardiner, D.M. (2008). Neurotrophic regulation of epidermal dedifferentiation during wound healing and limb regeneration in the axolotl (Ambystoma mexicanum). Dev. Biol. 319:321-325.
  • Ghosh, S., Roy, S., Seguin, C., Bryant, S.V. and Gardiner, D.M. (2008). Expression of Wnt5a and Wnt5b in developing and regenerating axolotl limbs. Dev. Growth & Diff. 50:289-297
  • Muneoka, K., Han, M., and Gardiner, D. (2008). Human Limb Regeneration: Understanding the Challenge. Scientific American 298:56-63.
  • Satoh, A., Gardiner, D.M., Bryant, S.V. and Endo, T. (2007). Nerve-induced ectopic limb blastemas in the axolotl are equivalent to amputation-induced blastemas. Dev. Biol. 312:231-244.
  • Gardiner, D.M. and Bryant, S.V. (2007). Homeobox-containing genes in limb regeneration. in “HOX Gene Expression” (ed. Spyros Papageorgiou). Landes Bioscience, Austin. 9 pp
  • Grun, F., Watanabe, H., Zamanian, Z., Maeda, L. Arima, K., Chubacha, R., Gardiner, D.M., Kanno, J., Iguchi, T., and Blumberg, B. (2006). Endocrine disrupting organotin compounds are potent inducers of adipogenesis in vertebrates. Mol Endocrinol. 20: 2141-2155.
  • Gardiner, D.M. and Susan V. Bryant (2006). Limb Regeneration. in “Fins into Limbs”, Brian Hall (ed.). Univ. Chicago Press. Chicago
  • Wong, B.J.F., Pandhoh, N., Truong, M.T., Diaz, S., Chao, K., Hou, S. and Gardiner, D.M. (2005). Identification of chondrocyte proliferation following laser irradiation, thermal injury, and mechanical trauma. Lasers in Surgery & Medicine. 37:89-96.
  • Gardiner, D.M. (2005). Ontogenetic decline of regenerative ability and the stimulation of human regeneration. Rejuvenation Res. 8:141-153.
  • Putta, S., Smith, J.J., Walker, J., Rondet, M., Weisrock, D.W., Monaghan, J., Samuels, A.K., Kump, K., King, D.C., Maness, N.J., Habermann, B., Tanaka, E., Bryant, S.V., Gardiner, D.M., Parichy, D.M. and Voss, S.R. (2004). From Biomedicine to Natural History Research: Expressed Sequence Tag Resources for Ambystomatid Salamanders. BMC Genomics 5:54-70.
  • Endo, T., Bryant, S.V., and Gardiner, D.M. (2004). A stepwise model system for limb regeneration. (2004). Dev. Biol. 270:135-145.