UCI Developmental & Cell Biology

Lan Huang, Ph.D.
Associate Professor

Nanjing University, China, B.S., Chemistry, July 1986

University of Florida, Ph.D., Analytical Chemistry, December 1995

D233 Medical Science I
University of California Irvine
Irvine, CA 92697

Tel: (949) 824-8548
Fax: (949) 824-8540
Email: lanhuang@uci.edu
Website: Lab Homepage

Mass spectrometry/proteomics - My lab is interested in developing and employing mass spectrometry-based proteomics approach to identify and characterize components of macromolecular machines and their posttranslational modifications, and map their interacting networks and structural topology in order to provide comprehensive information to elucidate the functions of these macromolecular machines. To this end, we have utilized the proteasome complex as our model system due to its enormous importance in health and medicine. In particular, we are interested in:

mapping the 26S proteasome interacting network to understand how polyubiquitinated substrates are recognized by and transport to 26S proteasome
identification and characterization of posttranslational modifications of 26S proteasome to understand their biological role in regulation of proteasome functions
characterization of proteomic profiling of the 26S proteasome complex at different physiological states
development of bioinformatics tools for analyzing mass spectral data to elucidate protein structural information

Recent Publications

Jones J, Wu K, Yang Y, Guerrero C, Nillegoda N, Pan ZQ, Huang L. A Targeted Proteomic Analysis of the Ubiquitin-Like Modifier Nedd8 and Associated Proteins. J Proteome Res. 2008 Feb 5; [Epub ahead of print] (featured article)
Wang X, Huang L. Identifying dynamic interactors of protein complexes by quantitative mass spectrometry. Mol Cell Proteomics. 2008 Jan;7(1):46-57. (Cover Paper)
Wang X, Guerrero C, Kaiser P, Huang L. Proteomics of proteasome complexes and ubiquitinated proteins. Expert Rev Proteomics. 2007 Oct;4(5):649-65. Review.
Wang X, Chen CF, Baker PR, Chen PL, Kaiser P, Huang L. Mass spectrometric characterization of the affinity-purified human 26S proteasome complex. Biochemistry. 2007 Mar 20;46(11):3553-65.
Guerrero C, Tagwerker C, Kaiser P, Huang L. An integrated mass spectrometry-based proteomic approach: quantitative analysis of tandem affinity-purified in vivo cross-linked protein complexes (QTAX) to decipher the 26 S proteasome-interacting network. Mol Cell Proteomics. 2006 Feb;5(2):366-78.