In Vitro Assays to Examine PD-1 Biology in Human T Cells

Our understanding of programmed cell demise 1 (PD-1) biology is proscribed as a result of technical difficulties in establishing reproducible, but easy, in vitro assays to check PD-1 signaling in main human T cells. The protocols on this article had been refined to check the implications of PD-1 ligation on short-term T cell signaling, long-term T cell perform, and the structural penalties of PD-1 ligation with PD-1 ligands. 

• Fundamental Protocol 1 addresses the necessity for a sturdy and reproducible short-term assay to look at the signaling cascade triggered by PD-1. We describe a phospho stream cytometry technique to find out how PD-1 ligation alters the extent of CD3ζ phosphorylation on Tyr¹⁴² , which might be simply utilized to different proximal signaling proteins.
  
  
• Fundamental Protocol 2 describes a plate-bound assay that’s helpful to look at the long-term penalties of PD-1 ligation comparable to cytokine manufacturing and T cell proliferation.
  
• Complementary to that, Fundamental Protocol Three describes an in vitro superantigen-based assay to judge T cell responses to therapeutic brokers focusing on the PD-1/PD-L axis, in addition to immune synapse formation within the presence of PD-1 engagement. Lastly, in Fundamental Protocol Four we define a tetramer-based technique helpful to interrogate the standard of PD-1/PD-L interactions.
  
• These protocols might be simply tailored for mouse research and different inhibitory receptors. They supply a beneficial useful resource to analyze PD-1 signaling in T cells and the purposeful penalties of varied PD-1-based therapeutics on T cell responses.
  
• © 2020 Wiley Periodicals LLC. Fundamental Protocol 1: PD-1 crosslinking assay to find out CD3ζ phosphorylation in main human T cells Fundamental Protocol 2: Plate-based ligand binding assay to check PD-1 perform in human T cells Help Protocol 1: T cell proliferation assay within the presence of PD-1 ligation Fundamental Protocol 3: In vitro APC/T cell co-culture system to judge therapeutic interventions focusing on the PD-1/PD-L1 axis Help Protocol 2: Microscopy-based strategy to judge the implications of PD-1 ligation on immune synapse formation Fundamental Protocol 4: Tetramer-based strategy to check PD-1/PD-L1 interactions.

Tailor-made Educating for Specialised (Para-)medical College students – Expertise From Incorporating a Related Genetic Illness All through a Course of Molecular Cell Biology

Worldwide, a compulsory course in Molecular Cell Biology is commonly a part of the (para-) medical curricula. Pupil audiences are commonly not receptive to such comparatively theoretical programs and academics typically battle to convey the mandatory data.

Right here, constructive expertise is shared on rigorously embedding a genetic illness that severely impacts the motion equipment, fibrodysplasia ossificans progressiva (FOP), in all points of a course for a global group of Analysis Grasp Human Motion Sciences college students. 

Varied molecular cell organic points of FOP had been systematically carried out within the course, protecting genetics, the biochemical penalties of the mutation, signaling pathways that have an effect on bone formation and lectures on methods to clone the mutation or treatment the mutation. College students had been invited to critically take into consideration methods to use the theories discovered within the course to investigate a analysis paper.

Through the sensible a part of the course, college students assisted in novel, innovative analysis on FOP affected person derived or management cells. Analysis findings had been reported in a analysis paper format. By constructing a Molecular Cell Biology course round an interesting illness, we managed to extend the overall motivation of the scholars for the course as mirrored in two particular questions of the course evaluations (p < 0.05).

It convincingly taught the relevance of a course of Molecular Cell Biology to college students with a main background in biomechanics and physiotherapy for his or her paramedical skilled life. This strategy of embedding an audience-tailored human illness with a recognized genetic trigger right into a course might be carried out to many medical curriculum associated programs and can improve college students’ notion of the relevance of a course. 

HK1 Rabbit pAb
A1054-100ul	Abclonal	100 ul	EUR 308

HK1 Rabbit pAb
A1054-200ul	Abclonal	200 ul	EUR 459

HK1 Rabbit pAb
A1054-20ul	Abclonal	20 ul	EUR 183

HK1 Rabbit pAb
A1054-50ul	Abclonal	50 ul	EUR 223

Rabbit HK1 ELISA Kit
ERTH0075	Abclonal	96Tests	EUR 521

HK1 antibody
70R-35878	Fitzgerald	100 ug	EUR 349
Description: Rabbit polyclonal HK1 antibody

HK1 Antibody
BF0126	Affbiotech	200ul	EUR 376
Description: HK1 antibody detects endogenous levels of total HK1.

HK1 Antibody
ABD6199	Lifescience Market	100 ug	EUR 438

HK1 antibody
70R-17747	Fitzgerald	50 ul	EUR 435
Description: Rabbit polyclonal HK1 antibody

HK1 antibody
70R-10229	Fitzgerald	50 ug	EUR 467
Description: Affinity purified rabbit polyclonal HK1 antibody

HK1 antibody
70R-10230	Fitzgerald	50 ug	EUR 467
Description: Affinity purified rabbit polyclonal HK1 antibody

HK1 antibody
10R-2226	Fitzgerald	100 ul	EUR 403
Description: Mouse monoclonal HK1 antibody

HK1 antibody
10R-2231	Fitzgerald	100 ul	EUR 403
Description: Mouse monoclonal HK1 antibody

HK1 Antibody
DF6199	Affbiotech	200ul	EUR 304
Description: HK1 Antibody detects endogenous levels of total HK1.

HK1 Antibody
CSB-PA230660-	Cusabio		EUR 335
Description: A polyclonal antibody against HK1. Recognizes HK1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

HK1 Antibody
CSB-PA230660-100ul	Cusabio	100ul	EUR 316
Description: A polyclonal antibody against HK1. Recognizes HK1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

HK1 Antibody
1-CSB-PA010468GA01HU	Cusabio	EUR 597.00 EUR 333.00	150ul 50ul
Description: A polyclonal antibody against HK1. Recognizes HK1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

HK1 Antibody
1-CSB-PA010468HA01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against HK1. Recognizes HK1 from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:1000-1:5000

HK1 Antibody
1-CSB-PA010468LA01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against HK1. Recognizes HK1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA

HK1 Antibody
1-CSB-PA002994	Cusabio	EUR 222.00 EUR 195.00	100ug 50ug
Description: A polyclonal antibody against HK1. Recognizes HK1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/20000

HK1 Antibody
1-CSB-PA093794	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Description: A polyclonal antibody against HK1. Recognizes HK1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:5000, IHC:1:50-1:200

HK1 Antibody
1-CSB-PA643791	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Description: A polyclonal antibody against HK1. Recognizes HK1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:5000, IHC:1:50-1:200

HK1 Antibody
32133-100ul	SAB	100ul	EUR 252

[KO Validated] HK1 Rabbit mAb
A0533-100ul	Abclonal	100 ul	EUR 459

[KO Validated] HK1 Rabbit mAb
A0533-200ul	Abclonal	200 ul	EUR 686

[KO Validated] HK1 Rabbit mAb
A0533-20ul	Abclonal	20 ul	EUR 221

[KO Validated] HK1 Rabbit mAb
A0533-50ul	Abclonal	50 ul	EUR 308

[KO Validated] HK1 Rabbit mAb
A0533	Abclonal	20 μL	EUR 15

Anti-HK1 antibody
PAab03893	Lifescience Market	100 ug	EUR 355

HK1 Polyclonal Antibody
A54597	EpiGentek	100 µg	EUR 570.55
Description: Ask the seller for details

HK1 Polyclonal Antibody
A54337	EpiGentek	100 µg	EUR 570.55
Description: Ask the seller for details

Anti-HK1 antibody
STJ24019	St John's Laboratory	100 µl	EUR 277
Description: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.

Anti-HK1 antibody
STJ110988	St John's Laboratory	100 µl	EUR 277
Description: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.

HK1/HK2 antibody
31975-100ul	SAB	100ul	EUR 252

HK1/HK2 antibody
31975-50ul	SAB	50ul	EUR 187

HK1 Monoclonal Antibody
27098-100ul	SAB	100ul	EUR 252

HK1 Monoclonal Antibody
27098-50ul	SAB	50ul	EUR 187

anti- HK1 antibody
FNab03893	FN Test	100µg	EUR 505.25
Description: Antibody raised against HK1

HK1 Conjugated Antibody
C32133	SAB	100ul	EUR 397

HK1 siRNA
20-abx919544	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

HK1 siRNA
20-abx919545	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

HK1 siRNA
20-abx902474	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

Rabbit Anti-HK1 monoclonal antibody, clone TU58-16
DCABH-4091	Creative Diagnostics	100 ul	EUR 777

HK1 Antibody, HRP conjugated
1-CSB-PA010468HB01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against HK1. Recognizes HK1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

HK1 Antibody, FITC conjugated
1-CSB-PA010468HC01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against HK1. Recognizes HK1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

HK1 Antibody, Biotin conjugated
1-CSB-PA010468HD01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against HK1. Recognizes HK1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

HK1 Antibody, HRP conjugated
1-CSB-PA010468LB01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against HK1. Recognizes HK1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

HK1 Antibody, FITC conjugated
1-CSB-PA010468LC01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against HK1. Recognizes HK1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

HK1 Antibody, Biotin conjugated
1-CSB-PA010468LD01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against HK1. Recognizes HK1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Hexokinase 1 (HK1) Antibody
20-abx125339	Abbexa	EUR 495.00 EUR 704.00 EUR 356.00	100 ul 200 ul 50 ul

Hexokinase 1 (HK1) Antibody
20-abx126932	Abbexa	EUR 411.00 EUR 592.00	100 ul 200 ul

Hexokinase 1 (HK1) Antibody
20-abx130172	Abbexa	EUR 425.00 EUR 133.00 EUR 1177.00 EUR 578.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Hexokinase 1 (HK1) Antibody
20-abx121415	Abbexa	EUR 300.00 EUR 439.00 EUR 189.00	100 ul 200 ul 30 ul

Hexokinase 1 (HK1) Antibody
20-abx109871	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Hexokinase 1 (HK1) Antibody
20-abx109872	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Hexokinase 1 (HK1) Antibody
20-abx112999	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul

Hexokinase 1 (HK1) Antibody
abx159534-100ul	Abbexa	100 ul	EUR 467

Hexokinase 1 (HK1) Antibody
20-abx214585	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

Hexokinase 1 (HK1) Antibody
20-abx214586	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

Hexokinase 1 (HK1) Antibody
abx233847-100ug	Abbexa	100 ug	EUR 481

Hexokinase 1 (HK1) Antibody
abx233893-100ug	Abbexa	100 ug	EUR 481

Hexokinase 1 (HK1) Antibody
20-abx172800	Abbexa	EUR 787.00 EUR 411.00	1 mg 200 ug

Hexokinase 1 (HK1) Antibody
20-abx329456	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

Hexokinase 1 (HK1) Antibody
abx332645-100ul	Abbexa	100 ul	EUR 425

Hexokinase 1 (HK1) Antibody
20-abx100798	Abbexa	EUR 398.00 EUR 133.00 EUR 1107.00 EUR 537.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug

Hexokinase 1 (HK1) Antibody
20-abx100799	Abbexa	EUR 398.00 EUR 133.00 EUR 1107.00 EUR 537.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug

Hexokinase 1 (HK1) Antibody
20-abx100800	Abbexa	EUR 411.00 EUR 133.00 EUR 1135.00 EUR 551.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug

Hexokinase 1 (HK1) Antibody
20-abx100801	Abbexa	EUR 411.00 EUR 133.00 EUR 1135.00 EUR 551.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug

Hexokinase 1 (HK1) Antibody
20-abx100802	Abbexa	EUR 411.00 EUR 133.00 EUR 1135.00 EUR 551.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug

Hexokinase 1 (HK1) Antibody
20-abx100803	Abbexa	EUR 425.00 EUR 133.00 EUR 1177.00 EUR 578.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Hexokinase 1 (HK1) Antibody
abx036294-100ug	Abbexa	100 ug	EUR 391

Hexokinase-1 (HK1) Antibody
20-abx137282	Abbexa	EUR 704.00 EUR 328.00 EUR 230.00	100 ug 20 ug 5 ug

Hexokinase 1 (HK1) Antibody
20-abx000977	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

Hexokinase 1 (HK1) Antibody
abx010915-100ul	Abbexa	100 ul	EUR 411

Hexokinase 1 (HK1) Antibody
abx015885-100ul	Abbexa	100 ul	EUR 411

Hexokinase 1 (HK1) Antibody
abx033903-400ul	Abbexa	400 ul	EUR 523

Hexokinase 1 (HK1) Antibody
abx033903-80l	Abbexa	80 µl	EUR 286

Hexokinase 1 (HK1) Antibody
abx033904-400ul	Abbexa	400 ul	EUR 523

Hexokinase 1 (HK1) Antibody
abx033904-80l	Abbexa	80 µl	EUR 286

HK1 Conjugated Monoclonal Antibody
C27098	SAB	100ul	EUR 397

HK1 Blocking Peptide
BF0126-BP	Affbiotech	1mg	EUR 195

HK1 Blocking Peptide
33R-4063	Fitzgerald	100 ug	EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of HK1 antibody, catalog no. 70R-10229

HK1 Blocking Peptide
33R-1787	Fitzgerald	100 ug	EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of HK1 antibody, catalog no. 70R-10230

HK1 Blocking Peptide
DF6199-BP	Affbiotech	1mg	EUR 195

anti-HK1 (7A7)
LF-MA30442	Abfrontier	100 ul	EUR 486
Description: Mouse Monoclonal to HK1

anti-HK1 (3A10)
LF-MA30447	Abfrontier	100 ul	EUR 486
Description: Mouse Monoclonal to HK1

HK1 cloning plasmid
CSB-CL010468HU-10ug	Cusabio	10ug	EUR 558
Description: A cloning plasmid for the HK1 gene.

pENTR223-HK1 vector
PVT11902	Lifescience Market	2 ug	EUR 308

Anti-HK1 (KO Validated) Antibody
A2125-100	Biovision	100 µl	EUR 465

Anti-Hexokinase 1/HK1 Antibody
A01504-1	BosterBio	100ug/vial	EUR 334

Focusing on A number of Myeloma by way of the Biology of Lengthy-Lived Plasma Cells

A number of myeloma (MM) is a hematological malignancy of terminally differentiated bone marrow (BM) resident B lymphocytes referred to as plasma cells (PC). PC that reside within the bone marrow embrace a definite inhabitants of long-lived plasma cells (LLPC) which have the capability to stay for very lengthy intervals of time (a long time within the human inhabitants).

LLPC biology is essential for understanding MM illness induction and development as a result of MM shares lots of the similar extrinsic and intrinsic survival applications as LLPC. Extrinsic survival alerts required for LLPC survival embrace soluble elements and mobile companions within the bone marrow microenvironment. Intrinsic applications that improve mobile constancy are additionally required for LLPC survival together with elevated autophagy, metabolic health, the unfolded protein response (UPR), and enhanced responsiveness to endoplasmic reticulum (ER) stress.

Focusing on LLPC cell survival mechanisms have led to straightforward of care therapies for MM together with proteasome inhibition (Bortezomib), steroids (Dexamethasone), and immunomodulatory medication (Lenalidomide). MM sufferers that relapse typically accomplish that by circumventing LLPC survival pathways focused by therapy. Understanding the mechanisms by which LLPC are capable of survive can enable us perception into the therapy of MM, which permits for the enhancement of therapeutic methods in MM each at analysis and upon affected person relapse.   

Ethylene glycol
GK7641-100ML	Glentham Life Sciences	100 ml	EUR 38

Ethylene glycol
GK7641-500ML	Glentham Life Sciences	500 ml	EUR 44

Ethylene glycol
GK7641-5L	Glentham Life Sciences	5 l	EUR 114

Ethylene glycol
GK7641-1L	Glentham Life Sciences	1 l	EUR 54

Ethylene glycol
ED0199	Bio Basic	250ml	EUR 58.7

2-Methylamino Thiourea
abx187868-100g	Abbexa	100 g	EUR 300

cDNA Synthesis SuperMix for qPCR
20-abx09801920ulSystems	Abbexa	EUR 690.00 EUR 565.00	100 rxns ×20 ul Systems 50 rxns × 20 ul Systems

Trifluoroacetic acid, Ultrapure for synthesis
GK2000-100ML	Glentham Life Sciences	100 ml	EUR 70

Trifluoroacetic acid, Ultrapure for synthesis
GK2000-1L	Glentham Life Sciences	1 l	EUR 293

1,1,3,3-Tetramethyl-2-thiourea
20-abx186112	Abbexa	EUR 272.00 EUR 1121.00	25 g 500 g

1,3-Diethyl-2-thiourea
GK7697-25G	Glentham Life Sciences	25 g	EUR 44

1,3-Diethyl-2-thiourea
GK7697-250G	Glentham Life Sciences	250 g	EUR 62

5-Bromovaleraldehyde Ethylene Acetal
20-abx186074	Abbexa	EUR 704.00 EUR 272.00 EUR 495.00	10 g 1 g 5 g

mono-(BOC)-ethylene diamine
20-abx184234	Abbexa	EUR 272.00 EUR 203.00 EUR 648.00	100 g 25 g 500 g

N-Boc-n'-methyl thiourea
20-abx185514	Abbexa	EUR 954.00 EUR 481.00	1 g 250 mg

Chlorobis(Ethylene)Rhodium(I)Dimer
20-abx188647	Abbexa	EUR 356.00 EUR 1191.00 EUR 565.00 EUR 871.00	100 mg 1 g 250 mg 500 mg

Poly(Ethylene Glycol) (N) Monomethacrylate
20-abx188846	Abbexa	EUR 370.00 EUR 217.00	100 g 25 g

Poly(ethylene glycol methyl ether)
abx181345-1000g	Abbexa	1000 g	EUR 342

Trans-1,2-Bis(Tributylstannyl)Ethylene
20-abx181364	Abbexa	EUR 244.00 EUR 1288.00 EUR 398.00	1 g 25 g 5 g

First-Strand cDNA Synthesis SuperMix for PCR
abx098018-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 578

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 718

Tri(Ethylene Glycol) Di-P-Toluenesulfonate
20-abx186587	Abbexa	EUR 286.00 EUR 230.00	25 g 5 g

OneScriptcDNA Synthesis Kit
G233	ABM	25 x 20 ul reactions	EUR 89

OneScriptcDNA Synthesis Kit
G234	ABM	100 x 20 ul reactions	EUR 141

OneScriptcDNA Synthesis SuperMix
G451	ABM	25 x 20 ul reactions	EUR 89

OneScriptcDNA Synthesis SuperMix
G452	ABM	100 x 20 ul reactions	EUR 141

cDNA Synthesis SuperMix
20-abx09801420ulSystems	Abbexa	EUR 565.00 EUR 481.00	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

Extraction Buffer for 2D Gel with Diluent, 50ml Urea, Thiourea and CHAPS
PL039	Bio Basic	50ml	EUR 72.62

OneScriptPlus cDNA Synthesis Kit
G235	ABM	25 x 20 ul reactions	EUR 97

OneScriptPlus cDNA Synthesis Kit
G236	ABM	100 x 20 ul reactions	EUR 169

OneScriptPlus cDNA Synthesis SuperMix
G453	ABM	25 x 20 ul reactions	EUR 97

OneScriptPlus cDNA Synthesis SuperMix
G454	ABM	100 x 20 ul reactions	EUR 169

circRNA cDNA Synthesis Kit
G627	ABM	25 rxn (20 ul/rxn)	EUR 309

CRISPR sgRNA Synthesis Kit
K1251-25	Biovision		EUR 403

Tetro cDNA Synthesis Kit
BIO-65042	Bioline	30 Reactions	Ask for price

Tetro cDNA Synthesis Kit
BIO-65043	Bioline	100 Reactions	Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65053	Bioline	50 Reactions	Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65053/S	Bioline	Sample	Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65054	Bioline	250 Reactions	Ask for price

Glutamine Synthesis stable cells
SC013	GenTarget	2 x 106 cell/ml x 1ml	EUR 1210
Description: Stable cell line expressing Glutamine Synthesis gene and RFP with Blasticidin resistance

Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column
FAPDE-holder-for-ion-exchange	Favorgen	1 prep	EUR 158

All-in-One First-Strand cDNA Synthesis SuperMix for PCR
abx098023-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 732

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)
CAS510A-KIT	SBI	1 Kit	EUR 805

Total-Transcriptome cDNA Synthesis Kit
G904	ABM	25 reactions	EUR 224

Total-Transcriptome cDNA Synthesis Kit
G905	ABM	100 reactions	EUR 544

Classic CRISPR sgRNA Synthesis Kit
K1252-25	Biovision	25 Reactions	EUR 439
Description: Classic CRISPR sgRNA Synthesis Kit

Express CRISPR sgRNA Synthesis Kit
K1253-25	Biovision		EUR 533

Molybdenum Cofactor Synthesis 2 Protein
20-abx263321	Abbexa	EUR 1609.00 EUR 328.00 EUR 230.00	100 ug 10 ug 2 µg

amfiRivert cDNA Synthesis Master Mix
R5101-050	GenDepot	50 rxns	EUR 415

amfiRivert cDNA Synthesis Master Mix
R5101-100	GenDepot	2X50 rxns	EUR 847

amfiRivert cDNA Synthesis Master Mix
R5101-200	GenDepot	4X50 rxns	EUR 1211

amfiRivet cDNA Synthesis 2X Buffer
R5102-050	GenDepot	500ul	EUR 134

amfiRivet cDNA Synthesis 2X Buffer
R5102-100	GenDepot	2x500ul	EUR 192

Mitomycin C (DNA synthesis inhibitor)
SIH-246-10MG	Stressmarq	10 mg	EUR 149
Description: The substance Mitomycin C is a dna synthesis inhibitor. It is synthetically produced and has a purity of >99%. The pure substance is dark purple crystalline solid which is May be dissolved in DMSO (15mg/mL); or water (1mg/mL, warm).

Mitomycin C (DNA synthesis inhibitor)
SIH-246-50MG	Stressmarq	50 mg	EUR 373
Description: The substance Mitomycin C is a dna synthesis inhibitor. It is synthetically produced and has a purity of >99%. The pure substance is dark purple crystalline solid which is May be dissolved in DMSO (15mg/mL); or water (1mg/mL, warm).

Accuris qMax cDNA Synthesis Kit
PR2100-C-100	BenchMark	1 PC	EUR 337.75

Accuris qMax cDNA Synthesis Kit
PR2100-C-25	BenchMark	1 PC	EUR 142.36

Accuris qMax cDNA Synthesis Kit
PR2100-C-250	BenchMark	1 PC	EUR 711.77

Accuris qMax cDNA Synthesis Kit
PR2100-C-S	BenchMark	1 PC	EUR 77.76

Custom Peptide Synthesis order form
Pepord-1	Alpha Diagnostics	1	Ask for price

Extraction Buffer for 2D Gel with Diluent, 50ml Urea, Thiourea, CHAPS and ASB-16
PL040	Bio Basic	50ml	EUR 115.25

Extraction Buffer for 2D Gel with Diluent, 50ml Urea, Thiourea, CHAPS and NDSB 201
PL042	Bio Basic	50ml	EUR 91.76

Poly(ethylene glycol) methyl ether (mPEG) Molecular Weight: 20000
PEG-20K	Alpha Diagnostics	100 mg	EUR 225

Extraction Buffer for 2D Gel with Diluent, 50ml Urea, Thiourea, CHAPS and SB 3-10
PL041	Bio Basic	50ml	EUR 91.76

All-in-One cDNA Synthesis SuperMix
B24403	Bimake	200 ractions	EUR 456
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix
B24408	Bimake	1000 ractions	EUR 1453
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

AzuraQuant cDNA Synthesis Kit - 25 Reactions
AZ-1995	Real Time Primers	25 Reactions	EUR 153

AzuraQuant cDNA Synthesis Kit - 100 Reactions
AZ-1996	Real Time Primers	100 Reactions	EUR 372

Recombinant Molybdenum Cofactor Synthesis 1 (MOCS1)
4-RPC623Hu01	Cloud-Clone	EUR 503.20 EUR 238.00 EUR 1612.00 EUR 604.00 EUR 1108.00 EUR 400.00 EUR 3880.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Molybdenum Cofactor Synthesis 1 expressed in: E.coli

Recombinant Molybdenum Cofactor Synthesis 1 (MOCS1)
4-RPC623Mu01	Cloud-Clone	EUR 458.40 EUR 226.00 EUR 1444.00 EUR 548.00 EUR 996.00 EUR 370.00 EUR 3460.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Molybdenum Cofactor Synthesis 1 expressed in: E.coli

EZClick? Global Protein Synthesis Assay Kit
K467-100	Biovision		EUR 533

Optimax First Strand cDNA Synthesis Kit
K4201100	Biochain	100 rnx	EUR 419

Molybdenum Cofactor Synthesis 1 (MOCS1) Antibody
20-abx131326	Abbexa	EUR 439.00 EUR 133.00 EUR 1233.00 EUR 592.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Molybdenum Cofactor Synthesis 1 (MOCS1) Antibody
20-abx131327	Abbexa	EUR 425.00 EUR 133.00 EUR 1205.00 EUR 578.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Molybdenum Cofactor Synthesis 2 (MOCS2) Antibody
20-abx113871	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul

Molybdenum Cofactor Synthesis 2 (MOCS2) Antibody
20-abx321401	Abbexa	EUR 439.00 EUR 328.00	100 ul 50 ul

Molybdenum Cofactor Synthesis 2 (MOCS2) Antibody
20-abx321402	Abbexa	EUR 439.00 EUR 328.00	100 ul 50 ul

Molybdenum Cofactor Synthesis 1 (MOCS1) Antibody
20-abx321947	Abbexa	EUR 439.00 EUR 328.00	100 ul 50 ul

Molybdenum Cofactor Synthesis 1 (MOCS1) Antibody
20-abx322547	Abbexa	EUR 439.00 EUR 328.00	100 ul 50 ul

Molybdenum Cofactor Synthesis 2 (MOCS2) Antibody
20-abx304514	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Molybdenum Cofactor Synthesis 2 (MOCS2) Antibody
abx235264-100ug	Abbexa	100 ug	EUR 481

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-20rxns20ulSystems	Abbexa	20 rxns × 20 ul Systems	EUR 732

Fly First-Strand cDNA Synthesis SuperMix
20-abx09885820ulSystems	Abbexa	EUR 718.00 EUR 578.00	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

Molybdenum Cofactor Synthesis 2 (MOCS2) Antibody
abx036894-100ug	Abbexa	100 ug	EUR 391

Molybdenum Cofactor Synthesis 1 (MOCS1) Antibody
abx037731-100ug	Abbexa	100 ug	EUR 391

Molybdenum Cofactor Synthesis 2 (MOCS2) Antibody
20-abx135733	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

Molybdenum Cofactor Synthesis 1 (MOCS1) Antibody
20-abx005887	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

Molybdenum Cofactor Synthesis 1 (MOCS1) Antibody
abx025653-400ul	Abbexa	400 ul	EUR 523

Molybdenum Cofactor Synthesis 1 (MOCS1) Antibody
abx025653-80l	Abbexa	80 µl	EUR 286

Molybdenum Cofactor Synthesis 2 (MOCS2) Antibody
abx028710-400ul	Abbexa	400 ul	EUR 523

Molybdenum Cofactor Synthesis 2 (MOCS2) Antibody
abx028710-80l	Abbexa	80 µl	EUR 286

amfiRivert II cDNA Synthesis Master Mix
R5500-050	GenDepot	50 rxns	EUR 374

amfiRivert II cDNA Synthesis Master Mix
R5500-100	GenDepot	2x50rxns	EUR 577

amfiRivert cDNA Synthesis Prime Master Mix
R5610-050	GenDepot	50 rxns	EUR 412